Ottenby, 030529. Notera bla gult ögonbrynsstreck (tydligt
framför ögat), gultonade örontäckare och strupe samt att ögonbrynsstrecket slutar innan
pannan typ nordsångare. Gröntonad ovan.
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"I have finally sequenced the blood sample in question, and I have
some
exciting news: It is almost certainly a P. (t.) nitidus!The genbank
sequence for nitidus was produced by Helbig et al.
(1995), which should certainly be reliable. The sample that I was
given definitely
contained DNA that matches the Helbig's nitidus sequence and is quite
different from all known viridanus sequences. Contamination is not a
possibility because I have not yet worked with nitidus samples in my new
lab. In Genbank there are nitidus and viridanus cytochrome b sequences
that have 701 base-pairs (bp) of overlap. I sequenced those 701 bp
from sample B135, both forward and backward. The sequences were very
clean. Out of those 701 bp, there is only one mismatch (a base that is
different, due to a point mutation) between B135 and the Genbank
nitidus. In contrast, there are 25 mismatches between B135 and the
Genbank viridanus. This translates into the following percent
divergences (raw DNA divergences):
B135 to nitidus: 0.14%
B135 to viridanus: 3.57%
nitidus to viridanus: 3.42%
Anyway, if the above was hard to follow, here is the take-home message: I
believe the results of this being a mistake are far below 1%, probably
more like 1/1000 or less. So go ahead and let people know."
Best wishes, Darren Irwin
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